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== <span style="color:blue">Part I: Pooling WGBS libraries & Normal Control WGBS libraries (01/16/2015)</span> == {| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8<\hiddentext> |- style="font-size:12pt" align="center" | width="81" colspan="4" height="15" | (The tube should be labeled ~2014-12-13) | align="center" width="17" | | width="213" colspan="4" | (The tube should be labeled ~2014-12-16) |- style="font-size:12pt" align="center" | height="15" | Sample | Conc. (ng/ul) | TruSeq Indx | Volume for 50ng (ul) | align="center" | | Sample | Conc. (ng/ul) | TruSeq Indx | Volume for 50ng (ul) |- style="font-size:12pt" align="center" | height="15" | PCP-9 | align="center" | 26.13 | align="center" | 8 | align="center" | 1.91 | align="center" | | NC-1 | align="center" | 13.73 | align="center" | 1 | align="center" | 3.64 |- style="font-size:12pt" align="center" | height="15" | PCP-4 | align="center" | 21.73 | align="center" | 3 | align="center" | 2.30 | align="center" | | NC-2 | align="center" | 16 | align="center" | 2 | align="center" | 3.13 |- style="font-size:12pt" align="center" | height="15" | 7P-6 | align="center" | 26.93 | align="center" | 22 | align="center" | 1.86 | align="center" | | NC-3 | align="center" | 16.93 | align="center" | 4 | align="center" | 2.95 |- style="font-size:12pt" align="center" | height="15" | PCP-8 | align="center" | 19.07 | align="center" | 7 | align="center" | 2.62 | align="center" | | NC-5 | align="center" | 13.07 | align="center" | 5 | align="center" | 3.83 |- style="font-size:12pt" align="center" | height="15" | PCP-6 | align="center" | 20.13 | align="center" | 5 | align="center" | 2.48 | align="center" | | NC-6 | align="center" | 15.33 | align="center" | 6 | align="center" | 3.26 |- style="font-size:12pt" align="center" | height="15" | PCP-7 | align="center" | 22.8 | align="center" | 6 | align="center" | 2.19 | align="center" | | NC-7 | align="center" | 13.73 | align="center" | 7 | align="center" | 3.64 |- style="font-size:12pt" align="center" | height="15" | PCP-2 | align="center" | 22.13 | align="center" | 1 | align="center" | 2.26 | align="center" | | NC-8 | align="center" | 15.87 | align="center" | 8 | align="center" | 3.15 |- style="font-size:12pt" align="center" | height="15" | PCP-3 | align="center" | 21.47 | align="center" | 2 | align="center" | 2.33 | align="center" | | NC-9 | align="center" | 16.53 | align="center" | 10 | align="center" | 3.02 |- style="font-size:12pt" align="center" | height="15" | 7P-1 | align="center" | 14.67 | align="center" | 18 | align="center" | 3.41 | align="center" | | NC-12 | align="center" | 14.8 | align="center" | 13 | align="center" | 3.38 |- style="font-size:12pt" align="center" | height="15" | PCP-5 | align="center" | 21.47 | align="center" | 4 | align="center" | 2.33 | align="center" | | NC-13 | align="center" | 13.19 | align="center" | 14 | align="center" | 3.79 |- style="font-size:12pt" align="center" | height="15" | 7P-8 | align="center" | 22.8 | align="center" | 25 | align="center" | 2.19 | align="center" | | NC-14 | align="center" | 14.93 | align="center" | 15 | align="center" | 3.35 |- style="font-size:12pt" align="center" | height="15" | 7P-3 | align="center" | 19.07 | align="center" | 20 | align="center" | 2.62 | align="center" | | NC-15 | align="center" | 13.6 | align="center" | 16 | align="center" | 3.68 |- style="font-size:12pt" align="center" | height="15" | 6P-1 | align="center" | 26 | align="center" | 9 | align="center" | 1.92 | align="center" | | NC-16 | align="center" | 13.87 | align="center" | 27 | align="center" | 3.60 |- style="font-size:12pt" align="center" | height="15" | 6P-8 | align="center" | 24.8 | align="center" | 14 | align="center" | 2.02 | align="center" | | NC-17 | align="center" | 13.47 | align="center" | 18 | align="center" | 3.71 |- style="font-size:12pt" align="center" | height="15" | 6P-10 | align="center" | 22.27 | align="center" | 16 | align="center" | 2.25 | align="center" | | NC-18 | align="center" | 14.67 | align="center" | 19 | align="center" | 3.41 |- style="font-size:12pt" align="center" | height="15" | 6P-9 | align="center" | 20.27 | align="center" | 15 | align="center" | 2.47 | align="center" | | NC-19 | align="center" | 13.47 | align="center" | 20 | align="center" | 3.71 |- style="font-size:12pt" align="center" | height="15" | 6P-2 | align="center" | 19.6 | align="center" | 10 | align="center" | 2.55 | align="center" | | NC-20 | align="center" | 12.79 | align="center" | 11 | align="center" | 3.91 |- style="font-size:12pt" align="center" | height="15" | 6P-3 | align="center" | 19.87 | align="center" | 11 | align="center" | 2.52 | align="center" | | NC-21 | align="center" | 12.31 | align="center" | 12 | align="center" | 4.06 |- style="font-size:12pt" align="center" | height="15" | 6P-4 | align="center" | 19.2 | align="center" | 12 | align="center" | 2.60 | align="center" | | NC-22 | align="center" | 16 | align="center" | 21 | align="center" | 3.13 |- style="font-size:12pt" align="center" | height="15" | 7P-10 | align="center" | 25.33 | align="center" | 27 | align="center" | 1.97 | align="center" | | NC-23 | align="center" | 13.6 | align="center" | 22 | align="center" | 3.68 |- style="font-size:12pt" align="center" | height="15" | 7P-7 | align="center" | 29.6 | align="center" | 23 | align="center" | 1.69 | align="center" | | NC-24 | align="center" | 11.97 | align="center" | 3 | align="center" | 4.18 |- style="font-size:12pt" align="center" | height="15" | 7P-2 | align="center" | 19.73 | align="center" | 19 | align="center" | 2.53 | align="center" | | NC-27 | align="center" | 14.67 | align="center" | 9 | align="center" | 3.41 |- style="font-size:12pt" align="center" | height="15" | 7P-5 | align="center" | 18 | align="center" | 21 | align="center" | 2.78 | align="center" | | NC-29 | align="center" | 12.96 | align="center" | 23 | align="center" | 3.86 |- style="font-size:12pt" align="center" | height="15" | 6P-5 | align="center" | 24.67 | align="center" | 13 | align="center" | 2.03 | align="center" | | NC-30 | align="center" | 15.47 | align="center" | 25 | align="center" | 3.23 |- style="font-size:12pt" align="center" | colspan="3" height="15" | Total Volume | align="center" | 55.83 | align="center" | | colspan="3" | Total Volume | align="center" | 84.70 |} :- Re-purified the two pools with 1X AMPure beads :- Resuspended each library with 33ul '''H2O''' :- Measure conc. with Qubit dsDNA HS assay using 1ul of purified library pool. Total volume left ~32ul ::- Normal WGBS library pool: conc. = 34.5 ng/ul --> total amount in 32ul = 1104 ng ::- Cancer patient WGBS library pool: conc. = 26.5 ng/ul --> total amount in 32ul = 848 ng :- The total amount of the two library pools is close to 1ug as recommended by User guide. :- Continue to SeqCap hybridization capture
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