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==Bisulfite Conversion of Jurkat gDNA== *Bisulfite conversion with zymo methylation gold *Convert 500ng of Jurkat gDNA (2 rxn, 100ng total) *Add 130ul of lighting conversion reagent to 5ul of jurkat gDNA and 15ul of ddH2O *Mix and centrifuge 1. 98°C for 8 minutes 2. 54°C for 60 minutes 3. 4°C hold *Add 600ul M-binding buffer to column *load the sample into column, mix by inverting column and centrifuge at 14000rpm for 30sec *discard flow through *add 100ul m-wash buffer to the column and centrifuge at 14000rpm for 30sec *add 200ul L-Desulphonation buffer to the column and sit at RT for 20 minutes and centrifuge at 14000rpm for 30sec *add 200ul of m-wash buffer to the column and centrifuge at 14000rpm for 30sec *Add 10ul of EBuffer to column sit for 5 mintues and centrifuge at 14000rpm for 30sec (2 rxn total)
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