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===Size selection using Invitrogen 2% SizeSelect gel=== *Fill any unused well with 30ul EB Buffer *Mix 15ul EB buffer with 0.5ul ladder in a 0.2ml tube *Load 20u end-polished DNA sample into two lanes(use 3 or more lanes if the starting DNA is more than 1ug) *Run the SizeSelect program for 12~14 min. Pause when the 125bp band just move into the middle collection well *Use pipette to extract all solution in each of the sample well, and quickly refill the wells with 20ul EB. *Resume the electrophoresis for additional 15sec, extract DNA and refill the wells with 20ul EB. *Resume the electrophoresis for additional 15sec, extract DNA (all the extracted DNA from the same sample are mixed in a tube) *Refill the wells with 20ul EB, and run the electrophoresis for additional 3 minutes. Take a picture of the gel to document the extracted fragment sizes. *Concentrate the DNA with a SpeedVac (no heat) to ~36ul (takes about 1hour).
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