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==Cytometer Setup== *Turn on the FACScan *Turn on the computer *Open both panels on the FACScan *Make sure the system is pressurized (Vent valve should be in the upwards position) (Sheath tank should be tight and stuck in place) (No gap between the sheath tank and the top of the rack) ===Purge bubbles from the sheath filter=== *Prepare a small waster beaker located near the sink *Pinch the tubing and remove white cap *Let drain into the small waste beaker until no bubbles are seen ===Purge bubbles from flow cell=== *Open up the middle panel on the cytometer, locate the flow cell (a small window with fluid in it) *Move the side arm to the side *Remove tube containing MilliQ H2O form the sample injection port (SIP) *Turn knob on the control panel to <Drain> in order to drain the flow cell (Notice the fluid level drops) *Once the flow cell is drained, turn the knob to <Fill> (Notice the fluid level rises) *Repeat the drain and fill procedure 3 times or until no bubbles are seen on the wall of the flow cell *Leave the control knob on <Fill> *Close the middle panel ===Purge bubble from SIP Line=== *Open the bottom panel *Locate the tubing linked to the orange valve leading to the waste tank (right) *Prepare test tube with MilliQ H2O *Open up the bottom panel *Reinstall the test tube with MilliQ H2O on the SIP and moving side arm back *Check wasteline (orange nozzle) for air bubbles (< 1 min) *Continue the filling function until tube is free of bubbles *Place cytometer on standby
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