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==Scanning Mix== '''Required Materials''' *Scanning Reagent *Scanning Buffer *Filter Unit *Scanning Mix Tube *Add the Scanning Reagent vial to the Scanning Buffer on ice *Rinse the empty vial into the Scanning Buffer three times with 1 ml Scanning Buffer *Invert the Scanning Buffer several times to mix *Leave on ice for 10 minutes *Invert several times to mix. Check to ensure that the solids are completely dissolved *Filter the Scanning Mix with the provided Scanning Mix Filter Unit *Pour 50 ml of the Scanning Mix into the 50 ml Scanning Mix Tube '''*If you are running more than 26 cycles on the Genome Analyzer, you must top off the Scanning Mix at the end of the first day of the sequencing run. The tube should be approximately one-third empty after running for six or seven hours''' *Record the weight of the reagent in the lab tracking worksheet *Leave the remaining solution in the Filter Unit at 4C for later cycles *Keep the Scanning Mix on ice until ready to load
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