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Andy Chen:Micromanipulation
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===General Protocol=== *Prepare pipette tips, tubes for collected samples, and a tube of medium (PBS, H2O, etc). UV everything 10-15 mins. *Transfer desired amount of medium to each sample tube (for example, fill tube with 1 μL if doing a subsequent 20 μL MDA amplification) *Load a tip into the pipetter and pre-load the tip with medium. To do this, move the pressure to slightly positive (+10 to +20), immerse the tip in the tube of medium, move the pressure to slightly negative (-5 to -10), wait for the pressure to equalize (it will stop clicking), then move the pressure back to zero. *Move the tip to the desired well. Take a picture of the fluorescence for reference. *Apply a positive pressure to eject some of the fluid into the well (enough to cover the bottom of the well). Move the tip around to help loosen the DNA-bead complex. Apply a negative pressure to extract the DNA. Check fluorescence to see if DNA was extracted adequately. If not, repeat ejection-extraction a few more times to collect as much DNA as possible. *Move tip to home and eject the collected DNA into sample tube by first applying a large positive pressure (+50, for example) and then hold the blue [INJECT] button for a few seconds to insure all the DNA was ejected.
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