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===Protocol=== Note: Reservation requires equipment authorization. * Sign in to the PEIIB log book. This will also be used to record pressure, power, time, and any malfunctions. * Check that the device is powered on. * The machine is stored under vacuum pressure. To open the lid, switch the VENT toggle to OPEN. * Open the lid and place the device on the forward half of the metal grating, about halfway between center and the edge. ** Plasma generation seems to be inhibited when samples are directly centered. * Close the lid and switch the VENT toggle to CLOSED. * Switch the SOL'N toggle to OPEN. This will start the vacuum. Wait until the TORR indicator reaches 45. This may take several minutes. ** The machine loses its calibration periodically. Anything under 50 is still OK for our purposes. * Switch the SOL'N toggle to CLOSED and switch the GAS 1 toggle to ON. Wait for the pressure to stabilize, then use the knob to adjust until the TORR indicator reads 200 +/- 5. ** Note: CW decreases pressure, CCW increases. * Switch ON the POWER toggle. Use the knob to adjust until the WATTS indicator reads 200 +/- 10. * Use the circular window on the front of the lid to check for plasma generation. We want a dark purple hue. * Wait 3 minutes for treatment. Monitor both the pressure and power indicators. * After 3 minutes, switch the POWER toggle to OFF, then switch the GAS 1 toggle to OFF. * Switch the VENT toggle to OPEN. Wait for the lid to pop up slightly; this indicates the pressure is equalized. * Open lid and remove sample. Close the lid and switch the SOL'N toggle to OPEN and wait for the TORR indicator to read 70. * Switch the SOL'N toggle to CLOSED. Record the run in the logbook.
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