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==Size Selection== '''Preparation''' Due to high concentration, each tube of 50ul was split into 3 tubes of ~16.3ul. 32.7ul of H2O was added to make a total volume of 50ul per tube. 50ul of 2x TBU Buffer was then added and the tubes were vortexed and spun down. The ladders were created by adding: -1.5ul 10bp ladder -9ul H2O -10ul 2x TBU Buffer Once the TBU gel was prepped, the samples were incubated at 75C for ~7min, then put on ice for ~1min. The ladders and samples were then loaded into the gel. After running the gels for 20min at 250V, they were stained with SYBR Gold. Upon viewing the gels through a UV stage, it was evident that the enzymatic digestion '''did not''' work: '''UV Cutting and Imaging''' [[Image:BCM_SizeSelection_1.jpg]] ''Gel Image 1'' [[Image:BCM_SizeSelection_2.jpg]] ''Gel Image 2''
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