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===results=== *performed RT with T20VN_PE_R, and PCR with (T7-top2-PCR-iaf, PCR_R.N2Ind[24] *nanodrop quant: {| {{table}} | align="center" style="background:#f0f0f0;"|'''Mg++''' | align="center" style="background:#f0f0f0;"|'''ng/ul''' |- | 9||67.11 |- | 10||61.81 |- | 11||10.53 |- | 12||11.56 |- | |} *nonamer at 42C has index22 *nonamer at 16C then 42C has index23 *Mg++ fragmentation shows a quality smear for both samples, which were successfully PCR amplified. according to the gel smears qPCr curves upon amplification *Mg++ samples showed weird curves. NTCs are correct, though could be something wrong with the SYBR green reading etc. nanodrop values after beads purification are fine, and gel smear is fine. *samples 9,10,11,12 are the Mg++ fragmentation samples. [[File:2012-06-22 qPCR after nonamer1, nonamer2 and mg++ run with plate-labeled.bmp|600px]] *TBE gel [[File:ZhangLab 2 2012-06-22 17hr 25min-labeled.jpg|600px]] *TBU gel of same samples shows smears still present with nonamers, but better fragmentation sizes for the Mg++ fragmentation [[File:ZhangLab 2 2012-06-22 18hr 41min-labeled.jpg|600px]] *proceeding to cloning and transformation to see if properly labeled fragments are obtained that contain genomic DNA.
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