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===Before starting protocols=== 1. Check if have enough reagents etc for the protocol *lysis buffer *nextera transposomes *transposase/transposome *RNA clean and concentrator *IVT reaction mixture *cells etc *blocking primer, RNase III, PolyA Polymerase, ATP, taq polymerase, other primers 2. samples this time: *description: all from male mice. E13.5 PGC samples from GW Na-Bi sequencing originally. KO's are for Tet1. cells were sorted by FACS into respective tubes. about 6 months old (from September 2012) {| {{table}} | align="center" style="background:#f0f0f0;"|'''smpl_ID''' | align="center" style="background:#f0f0f0;"|'''smpl_rep''' | align="center" style="background:#f0f0f0;"|'''sex''' | align="center" style="background:#f0f0f0;"|'''geno''' | align="center" style="background:#f0f0f0;"|'''cell_type''' | align="center" style="background:#f0f0f0;"|'''cell_num''' | align="center" style="background:#f0f0f0;"|'''''' | align="center" style="background:#f0f0f0;"|'''volume''' |- | 1-||1||male||WT||somatic|| 62,459 ||2x 10K||~100 uL |- | 3-||2||male||WT||somatic|| 27,813 ||2x 1K||~70 uL |- | 1+||1||male||WT||PGC|| 8,213 ||2x 1K||~10 uL |- | 3+||2||male||WT||PGC|| 2,495 ||---||~20 uL |- | 5-||1||male||-/-||somatic|| 34,924 ||2x10K||~100 uL |- | 8-||2||male||-/-||somatic|| 32,766 ||2x1K||~100 uL |- | 5+||1||male||-/-||PGC|| 5,888 ||2x1K||~20 uL |- | 8+||2||male||-/-||PGC|| 1,876 ||---||~8 uL |- | |} Samples: Index 61, N2 adptr, 1. 1- WT somatic 10K cells rep1 Index 62, N2 adptr, 2. 1- WT somatic 10K cells rep2 Index 63, N2 adptr, 3. 3- WT somatic 1K cells rep1 Index 64, N2 adptr, 4. 3- WT somatic 1K cells rep2 Index 65, N2 adptr, 5. 3+ WT PGC 1K cells rep1 (pretty sure used 1+) Index 66, N2 adptr, 6. 3+ WT PGC 1K cells rep2 (pretty sure used 1+) Index 67, N2 adptr, 7. 5- KO somatic 10K cells rep1 Index 68, N2 adptr, 8. 5- KO somatic 10K cells rep2 Index 69, N2 adptr, 9. 8- KO somatic 1K cells rep1 Index 70, N2 adptr, 10. 8- KO somatic 1K cells rep2 Index 71, N2 adptr, 11. 5+ KO PGC 1K cells rep1 Index 72, N2 adptr, 12. 5+ KO PGC 1K cells rep2 Index 60, N2 adptr, 13. NTC
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