Editing
CES36k18bp
(section)
Jump to navigation
Jump to search
Warning:
You are not logged in. Your IP address will be publicly visible if you make any edits. If you
log in
or
create an account
, your edits will be attributed to your username, along with other benefits.
Anti-spam check. Do
not
fill this in!
==Size Selection and Extraction== '''Size Selection''' Based on the Nanodrop readings, I used this set as a test for how to divide all the sets. I divided each tube of 50ul into two tubes of 25ul and added 25ul 2x TBU Buffer, making 50ul total for each gel. The samples were run against a 10bp ladder. Each gel was then cut to excise the band at ~100bp. The first gels run were slightly overloaded, but the second gels were clean: [[image: CES_Test.jpg]] ''First set of CES gels'' [[image: CES_2.jpg]] ''Second set of CES gels'' After excising the correct band from the gel, each band was cut in half. These halves were placed into shearing tubes (a 0.5mL tube with a hold poked through the bottom resting freely inside a 1.6mL tube) and spun down at 14,000 rpm for 1:30. '''Extraction''' After the gel fragments were sheared, the 0.5mL tubes were tossed and 400ul of 1x TE Buffer was added to each tube. Each tube was then placed in a 37C incubator and placed on a spinner/vortexer for approximately 1 hour. After this each tube was run through another round of ethanol purification. The ethanol products were suspended in 5ul of H2O and collected into a fresh 1.6mL tube.
Summary:
Please note that all contributions to ZhangLabWiki may be edited, altered, or removed by other contributors. If you do not want your writing to be edited mercilessly, then do not submit it here.
You are also promising us that you wrote this yourself, or copied it from a public domain or similar free resource (see
ZhangLabWiki:Copyrights
for details).
Do not submit copyrighted work without permission!
Cancel
Editing help
(opens in new window)
Navigation menu
Personal tools
Not logged in
Talk
Contributions
Create account
Log in
Namespaces
Page
Discussion
English
Views
Read
Edit
View history
More
Search
Navigation
Main Page
Current events
Recent changes
Random page
Investigators
Matt Cai
Song Chen
Eric Chu
Dinh Diep
Elizabeth Duong
Shicheng Guo
Alan Fung
Daniel Jacobsen
Blue Lake
Huy Lam
Alice Li
Andrew Richards
Brandon Sos
Chris Wei
Yan Wu
Kun Zhang
Tools
What links here
Related changes
Special pages
Page information