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==Test 1== We found binder clips of an appropriate size to hold in the stoppers. This lets us use the higher pressures necessary for running the oil at low temperature (~10 psi). We also used a new setup, which is shown below. The new setup incorporates an ice bath for the reagents as well as the chip (on a block). It is then run across a hot plate at ~37-40C, across a light microscope for viewing. Finally, the solution is deposited in tubes waiting in the rock bath, also set at 37C. [[Image:MicrofluidicSetup_20160314.jpg|800px]] We tried the VA-044 again using the [[Daniel:Notebook/ComboLock/Polyacrylamide#VA-044 (Tissue)|standard recipe]]. ===Run Parameters=== *Line 1: Oil (on ice); 12 psi; ~2 mL *Line 2: Aqueous (CLARITY; on ice); 2 psi; ~1 mL ===Results=== We did get droplet formation, although the droplets were aqueous and could easily be popped using a pipette tip or needle. Therefore, we went back to the drawing board and realized that the acrylamide concentration we were using was too low. The CLARITY solution uses PFA to crosslink the proteins and nucleotides to the hydrogel matrix. This means that without any cellular material we should use extra acrylamide to create stiffer gels.
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