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===Results=== '''PCR Gel Image''' [[Image:2016-06-24-CombolockTest-Stage2-FIXED.png|500px]] I had to fix the lane annotations from the top annotations, which were the originals. I forgot (see above) that I switched lanes 1 and 5, so that definitely isn't corrected on the gel. Additionally, I believe that lanes 5-8 were put in the gel backwards (long story involving PCR machines). So, fixing those you get the black box labeling, which I believe to be correct. If that is correct, the 48 bp backbone padlock doesn't work, and it looks like the 68 works better than the 88 (qualitatively). So in the future I'd recommend around a 20 bp spacer, as is used in the 68 bp backbone version. Since I have results, I want to go ahead with size selection and sequencing. [[Category:ComboLock]] [[Category:20160623]]
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