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===Results=== {| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> |- style="font-size:12pt" align="center" | align="center" width="65" height="30" | |style="font-weight:bold" width="65" | ng/uL DNA |style="font-weight:bold" width="65" | pmol/uL dye |style="font-weight:bold" width="65" | ug DNA in 20 uL |style="font-weight:bold" width="71" | pmol/uL DNA |style="font-weight:bold" width="71" | dye/probe |style="font-weight:bold" width="71" | dye/100 bp |style="font-weight:bold" width="65" | Base:Dye Ratio (:1) |- style="font-size:12pt" align="center" |style="font-weight:bold" height="15" | AmpR9 | align="center" | 64.6 | align="center" | 0.6 | align="center" | 1.3 | align="center" | 3.9 | align="center" | 0.2 | align="center" | 0.3 | align="center" | 326 |- style="font-size:12pt" align="center" |style="font-weight:bold" height="15" | AmpR10 | align="center" | 71.1 | align="center" | 0.4 | align="center" | 1.4 | align="center" | 4.3 | align="center" | 0.1 | align="center" | 0.2 | align="center" | 539 |} It would seem that I can always tell beforehand whether a yield will be good or bad, by the color of the solution post incubation. If the solution has a faint green color it will be a good yield. If the solution remains clear it will be a bad yield. Therefore I'm going to ignore columns for future trials (yes, it looks like I need more troubleshooting). That will save on time and $$, and hopefully I'll get to the bottom of this before long.
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