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===Labeling Results=== {| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext> |- style="font-size:12pt;font-weight:bold" align="center" | width="65" height="30" | Sample | width="65" | ng/uL dsDNA | width="65" | pmol/uL dye | width="71" | pmol/uL ds probe | width="71" | dye/100bp | width="65" | ng/uL ssDNA | width="65" | pmol/uL dye | width="71" | pmol/uL ss probe | width="71" | dye/100bp |- style="font-size:12pt" align="center" |style="font-weight:bold" height="30" | Alu2D-Pre Column | align="center" | 170 | align="center" | 115 | align="center" | | align="center" | | align="center" | 113.2 | align="center" | 116.6 | align="center" | | align="center" | |- style="font-size:12pt" align="center" |style="font-weight:bold" height="30" | Blank-Pre Column | align="center" | 157.4 | align="center" | 111 | align="center" | | align="center" | | align="center" | 103.3 | align="center" | 110.6 | align="center" | | align="center" | |- style="font-size:12pt" align="center" |style="font-weight:bold" height="30" | Cot1-Pre Column | align="center" | 150.9 | align="center" | 105 | align="center" | | align="center" | | align="center" | 102.1 | align="center" | 106.4 | align="center" | | align="center" | |- style="font-size:12pt" align="center" |style="font-weight:bold" height="30" | V6S3-Pre Column | align="center" | 160.3 | align="center" | 109.2 | align="center" | | align="center" | | align="center" | 109.6 | align="center" | 112.9 | align="center" | | align="center" | |- style="font-size:12pt" align="center" |style="font-weight:bold" height="30" | Alu2D-Post Column | align="center" | 24.4 | align="center" | 0.5 | align="center" | 0.4 | align="center" | 1.4 | align="center" | 12.6 | align="center" | 0.5 | align="center" | 0.4 | align="center" | 1.3 |- style="font-size:12pt" align="center" |style="font-weight:bold" height="30" | Blank-Post Column | align="center" | 1 | align="center" | 0 | align="center" | 0.0 | align="center" | 0.0 | align="center" | 0.2 | align="center" | 0 | align="center" | 0.0 | align="center" | 0.0 |- style="font-size:12pt" align="center" |style="font-weight:bold" height="30" | Cot1-Post Column | align="center" | 25.9 | align="center" | 1.7 | align="center" | 0.2 | align="center" | 4.3 | align="center" | 15.6 | align="center" | 1.3 | align="center" | 0.3 | align="center" | 2.8 |- style="font-size:12pt" align="center" |style="font-weight:bold" height="30" | V6S3-Post Column | align="center" | 41.3 | align="center" | 0.6 | align="center" | 0.8 | align="center" | 1.0 | align="center" | 24.4 | align="center" | 0.3 | align="center" | 0.9 | align="center" | 0.4 |} This is a big table, but there are a couple of messages to take away from this. *Excess dye causes a false DNA reading. It doesn't appear to have an effect when dye concentration is low, but when the dye concentration was high (pre-spin column), even the blank sample measured a considerable amount of DNA. I'd recommend always using a blank column and subtracting the background from future samples if pre-purification measurements are needed. *The Cot1 DNA is reproducibly eluting with dye. Given the other labeling results from previous days ([[Daniel:Notebook/HiResChrPaint/2014-1-13|1/13/2014]] and [[Daniel:Notebook/HiResChrPaint/2014-1-10|1/10/2014]]), I'd say that the Cot1 DNA, although having no amine groups, might be being labeled anyway. Or, at least, it's clogging up the column and allowing the dye to co-elute.
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