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Dinh:Probes Prep May2015
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===Consumed reagents=== * For future reference to calculate total cost of probes prep for 1.5x96 wells 6,600 uL KAPA Sybr Fast 2xMM -- $347/10 mL 900 uL QIAEX II silica beads -- $169/1.5 mL 180 uL Lambda Exo (5 U/uL) -- $209/1.0 mL 210 uL USER (NEB) -- $216/.250 mL 74 uL DpnII (50 U/uL) -- $211/.100 mL 12X ssDNA/RNA cleanup & concentrate columns -- $149/50X * Total amount above exclude size selection =$ XXX * 200 ng gDNA at 200:1 probes to target ratio uses 314.29 ng of probes. * Noi's yield is 7-8 ug per plate, Alan's yield is 12-16 ug per plate. * If I produce ~6ug of probes, each sample will cost ~ $ XXX in probes (excludes size selection materials).
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