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Dinh:Protocols/Probes Prep Oct6
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==Step 2: Ethanol Precipitation== 1. Pool all reactions in a plate into Solution Basin (55ml) 2. Add: ** NaOAc (3M), 260 ul ** GlycoBlue, 8ul ** Ethanol 100%, 6ml 3. Transfer to 15 ml tube. Store at -70C for more than 20 min 4. Turn on centrifuge (might need to switch the "swirlers") 5. Spin at 3700rpm->20min->4C 6. Remove all supernatant, leave the blue pellet 7. Wash with cold ethanol (75%) ~750ul 8. Move pellet to 1.6ml tube, vortex/mix well 9. Spin at 10,000rpm->5min->4C 10. Remove all supernatan, leave 30 min to air dry. 11. Resuspend DNA with 125 ul dH20 12. Incubate at 37C for 30 min. 13. Resuspend by pipetting. Transfer to 200 ul PCR tubes/strip.
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