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====Result==== *0~30% Formamide concentration showed similar number of rolony, but the number decreased at 70%. *Need to try 20%, 30%, 40%, twice at each time in order to confirm 30% is maximum condition! =====1st Rolony Count===== *Before Step 0 (At the beginning Cy3 probe were annealed) --> 5932 *Step 1 : Cy5 in 2x SSC --> 5469 *Step 3 : Cy5 in 70% Formamide + 2x SSC --> 543 *Step 5 : Cy5 in 10% Formamide + 2x SSC --> 5413 *Step 7 : Cy5 in 30 % Formamide + 2x SSC --> 5446 *Step 9 : Cy5 in 50% Formamide + 2x SSC --> 4793 *Step 11 : Cy5 in 70% Formamide + 2x SSC --> 421 {| {{table}} border = 1 | align="center" style="background:#f0f0f0;"|'''Formamide (%)''' | align="center" style="background:#f0f0f0;"|'''1st Rolony Count''' |- | align="center" | 0 || align="center" | 5469 |- | align="center" | 10 || align="center" | 5413 |- | align="center" | 30 || align="center" | 5446 |- | align="center" | 50 || align="center" | 4793 |- | align="center" | 70 || align="center" | 482 |} *0% : average from 2 results =====Number of 1st Rolony vs. Formamide %===== [[File:Chart_DyeProbeTest_Cy5-1stRolony_Step1-3-5-7-9-11.png|850px]] =====1st Rolonies along each step===== [[File:DyeProbeTest_Cy5-1stRolony_Step1-3-5-7-9-11_label.png|850px]] *'''Raw images''' **[[Media:DyeProbeTest_MatTek20130709_1stRolony_Cy3_2k_600Hz_ch00_MIP.tif|Before Step0 (Cy3, ]][[Media:DyeProbeTest_MatTek20130709_1stRolony_Cy3_2k_600Hz_ch01_MIP.tif|BF)]], **[[Media:DyeProbeTest.lif_MatTek20130709_Step1_Cy5_ch00_MIP.tif|Step1 (Cy5]], [[Media:DyeProbeTest.lif_MatTek20130709_Step1_Cy5_ch01_MIP.tif|BF)]], [[Media:DyeProbeTest.lif_MatTek20130709_Step3_Cy5-Formamide70_Cy5_ch00_MIP.tif|Step3]], [[Media:DyeProbeTest.lif_MatTek20130709_Step5_Cy5-Formamide10_Cy5_ch00_MIP.tif|Step5]], [[Media:DyeProbeTest.lif_MatTek20130709_Step7_Cy5-Formamide30_Cy5_ch00_MIP.tif|Step7]], [[Media:DyeProbeTest.lif_MatTek20130709_Step9_Cy5-Formamide50_Cy5_ch00_MIP.tif|Step9 (Cy5, ]][[Media:DyeProbeTest.lif_MatTek20130709_Step9_Cy5-Formamide50_Cy5_ch01_MIP.tif|BF)]], [[Media:DyeProbeTest.lif_MatTek20130709_Step11_Cy5-Formamide70_Cy5_ch00_MIP.tif|Step11]] *'''Stage was moveed a little from Step9 by mistake, so the location was re-configured.'''
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