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===CircLigase II reaction on the dish=== *based on Debugging plan ([[Hosuk:LabNotes/2013-5-1|05/01]]) *Reaction for 2.5 hr *Aspirate and transfer solution from dish to tube *Total volume collected **8~9uL from the sample at wet towel **about 50uL from the sample at mineral oil, but this volume include oil. After spinning the actual reaction solution seemed 8~10uL *Run Exo I reaction **9uL of pre-circulated DNA + 1uL of Exo I **6uL of wet towel sample + 1uL of Exo I **6uL of mineral oil sample + 1uL of Exo I **45min at 37C --> 15min at 80C =====Result===== *CircLigase II reaction worked on a dish well *Wet towel method looked better efficiency than oil cover method did. *[[File:GelTable_CircLigaseIIonDish_2.jpg|550px]] *[[File:CircOnDish_2_edit.jpg|350px]] ---- ----
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