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=====Image processing procedure===== **Take raw images data to storage server. Raw images from Confocal is 12 bit **Open raw images with Imaris, and export to Huygens **Deconvolution with Huygens, end export back to Imaris (become 32 bit) **Check images with Imaris, and reduce image size ***Maximum projection --> 2D image from 3D z stack images ***Downsampling to 8 bit ***Reduce resolution from 4000 x 4000 to 1000 x 1000 **Link time series **Create mask for aligning with Imaris and align images with Huygens **Convert time to z --> Get one image has pixel towers (stacks) of each time series of each individual rolony signal **Use genome libraries to match each colors of rolonies to known libraries, without concerning further image processing to remove noise, background and resolve which pixel is real or not.
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