Editing
Hosuk:LabNotes/2013-9-30
(section)
Jump to navigation
Jump to search
Warning:
You are not logged in. Your IP address will be publicly visible if you make any edits. If you
log in
or
create an account
, your edits will be attributed to your username, along with other benefits.
Anti-spam check. Do
not
fill this in!
====Procedure==== *strip previous proves, *rehybridize Cy5 labeled probe for 1st rolonies, and ATTO 488 labeled probe for ACTB target *1uL of 200uM ATTO488-ACTBTargetOn1stRolony + 2uL of 100uM Cy5-1stRolony in 197uL 2x SSC *Pre-heat probe mix at 60C for 5min, and add the probe mix to two dishes - 0623 3uL Phi29(used ACTB, RAB7A Padlock probing and created 2nd Rolony), and 0623 10uL Phi29 (only 1st Rolonies) *incubate at RT for 10min, and wash with 2x SSC twice, store in PBS, and image with Confocal
Summary:
Please note that all contributions to ZhangLabWiki may be edited, altered, or removed by other contributors. If you do not want your writing to be edited mercilessly, then do not submit it here.
You are also promising us that you wrote this yourself, or copied it from a public domain or similar free resource (see
ZhangLabWiki:Copyrights
for details).
Do not submit copyrighted work without permission!
Cancel
Editing help
(opens in new window)
Navigation menu
Personal tools
Not logged in
Talk
Contributions
Create account
Log in
Namespaces
Page
Discussion
English
Views
Read
Edit
View history
More
Search
Navigation
Main Page
Current events
Recent changes
Random page
Investigators
Matt Cai
Song Chen
Eric Chu
Dinh Diep
Elizabeth Duong
Shicheng Guo
Alan Fung
Daniel Jacobsen
Blue Lake
Huy Lam
Alice Li
Andrew Richards
Brandon Sos
Chris Wei
Yan Wu
Kun Zhang
Tools
What links here
Related changes
Special pages
Page information