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==PCR== Template 10ul x4 2X iProof Mastermix 50ul 200ul AmpF6.2SoL (10uM) 4ul 16ul AmpR6.2SoL (10uM) 4ul 16ul 50X SYBG I 0.4ul 1.6ul H2O 31.6ul 126.4ul I did 20 cycles for BJ and 21 cycles for Hybrid_1. [[Image:20090615_CES27k9bp_Hybrid1_BJ.jpg]]20090615_CES27k9bp_Hybrid1_BJ Qiaquick purification and gel quantification: Hybrid_1: 8.6ng/ul x 30ul BJ: 14.3ng/ul x 30ul I send the two samples to Zhen Ye as follows: Hybrid_1: 4.3ng/ul (37nM) x 5ul BJ: 7.2ng/ul (62nM) x 5ul
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