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==PCR== Template 10ul x9 2X iProof Mastermix 50ul 450ul AmpF6.3SoL (10uM) 4ul 36ul AmpR6.3SoL (10uM) 4ul 36ul 50X SYBG I 0.4ul 3.6ul H2O 31.6ul 286.2ul [[Image: 20090622_cpg97k_A subset_No09_1~7.jpg| 20090622_cpg97k_A subset_No09_1~7.jpg]]20090622_cpg97k_A subset_No09_1~7 [[Image: 20090622_cpg97k_B subset_No09_1~7.jpg]]20090622_cpg97k_B subset_No09_1~7 Egel purify the A subset captured targets. qiaquick purify the B subset captured targets. Gel quantification: No09_1_A: 1.6 ng/ul No09_1_B: 4.6 No09_2_A: 1.57 No09_2_B: 3.42 No09_3_A: 0.94 No09_3_B: 4.47 No09_4_A: 2.42 No09_4_B: 2.31 No09_5_A: 2.31 No09_5_B: 3.85 No09_6_A: 1.48 No09_6_B: 4.85 No09_7_A: 1.43 No09_7_B: 2.87 mix the A and B subsets to 1:1 mole ratio. send half of the samples to Billy Li on 09-06-24. No09_1_Mix_cpg97k 2.35ng/ul, 6.0nM x 40ul No09_2_Mix_cpg97k 2.19ng/ul, 5.8nM x 30ul No09_3_Mix_cpg97k 1.53ng/ul, 4.1nM x 48ul No09_4_Mix_cpg97k 2.37ng/ul, 6.3nM x 30ul No09_5_Mix_cpg97k 2.93ng/ul, 7.8nM x 25ul No09_6_Mix_cpg97k 2.32ng/ul, 6.2nM x 40ul No09_7_Mix_cpg97k 1.91ng/ul, 5.1nM x 30ul I got the Covaris sheared samples back on 09-06-24. The image on the gel: [[Image:20090624_samples after Covaris shearing.jpg]]NA21687*1 062507 (No09_1) after Covaris shearing The sheared DNA were not used due to the possible defects of Covaris shearing in Billy's lab. ===redo PCR with V6.3NH2 primer(2009-07-24)=== I redo the PCR with V6.3NH2 primer and 2x Master mix (NEB) and purified the amplicons with Qiaquick column. No09_1_Mix_cpg97k 920ng, 100ul No09_2_Mix_cpg97k 610ng, 100ul No09_3_Mix_cpg97k 780ng, 100ul No09_4_Mix_cpg97k 680ng, 100ul No09_5_Mix_cpg97k 720ng, 100ul No09_6_Mix_cpg97k 1260ng, 100ul No09_7_Mix_cpg97k 770ng, 100ul
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