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==DNA shearing== *I sheared three samples (Cell-a, Cell-b, gDNA-a) with nebulizers at 35psi for 6 min. *The sheared DNA (~600ul) were precipitated with 1V isopropanol. *The recovery was pretty poor. Also the sheared DNA fragments are still too big (500bp-1.2kb). *I decided to send all the amplicons (5 S1 treated, and 11 untreated) to Covaris for shearing. The target size is 300bp. GM18507A Purified DNA (50ul) + 50ul 1X TE GM18507B Purified DNA (50ul) + 50ul 1X TE GM18507C Unpurified DNA (50ul) + 50ul 1X TE GM18507D S1 treated DNA (40ul) + 50ul 1X TE GM18507E Unpurified DNA (50ul) + 50ul 1X TE GM18507F Unpurified DNA (50ul) + 50ul 1X TE GM18507G S1 treated DNA (40ul) + 50ul 1X TE GM18507H Unpurified DNA (50ul) + 50ul 1X TE GM20431A Purified DNA (50ul) + 50ul 1X TE GM20431B S1 treated DNA (40ul) + 50ul 1X TE GM20431C Unpurified DNA (50ul) + 50ul 1X TE GM20431D Unpurified DNA (50ul) + 50ul 1X TE GM20431E Unpurified DNA (50ul) + 50ul 1X TE GM20431F Unpurified DNA (50ul) + 50ul 1X TE GM20431G S1 treated DNA (40ul) + 50ul 1X TE GM20431H Unpurified DNA (50ul) + 50ul 1X TE
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