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===Whole cell lysates=== *Yesterday I collected ~3 millions GM20431 cells (442,000/ml x 6.8ml), and lysed them with 200ul Cell Lysis Buffer (20M EDTA, 10mM Tris.HCl , 200mM NaCl, 0.2% Triton X-100, 0.1AU/ml Qiagen Protease). *The resulting cell lysate is extremely sticky. I added 800ul EB buffer, still quite sticky. The DNA concentration is roughly 18ug per ml or 18ng/ul. *Take 20ul, add 100 UV-treated water -> 3ng/ul; *Make 1:10 serial dilution to 300pg/ul, 30pg/ul, 3pg/ul and then 1:5 dilution to 0.6pg/ul. Pipette slowly to avoid breaking HMW DNA.
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