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====Second Strand cDNA synthesis==== *Follow NEB E6111S protocol: [https://www.neb.com/protocols/1/01/01/second-strand-cdna-synthesis-e6111] *Added 48 ul H20 to each tube *Added 8ul 10x Second strand synthesis reaction buffer *Added 4ul Second strand synthesis enzyme mix *Mixed by pipetting *Incubated for 2.5 hours at 16C *Purified using Qiagen minelute column (eluted 23ul into 1.5ml tube labelled HBRR cDNA) *Measured DNA conc with Nandrop: **43ul at 63.7ng/ul (2.74 ug) sample left in each tube **"High yield" because RNA was not digested and went from single stranded to double stranded; slightly higher but consistent with last time [http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-3-9 2013-3-9]
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