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===RT_RAB7A Primers and the Number of RAB7A Primary Rolonies=== *Using RAB7A_1stRolonyFISH (ATTO550) in column 10 {| {{table}} | align="center" style="background:#f0f0f0;"|'''Well''' | align="center" style="background:#f0f0f0;"|'''RT primer''' | align="center" style="background:#f0f0f0;"|'''Count''' |- | C10||220nt||24 |- | D10||300nt||24 |- | E10||510nt||13 |- | F10||1000nt||35 |- | F3||RandomHex||5 |} ====Including Hosuk's Results [[Hosuk:LabNotes/2013-12-30#Result]]==== *Combining my results ([[Matt:LabNotes/2014-1-28#Results]]) with Hosuk's **Hosuk didn't see any rolonies using RAB7A_1stRolonyFISH [[File:RTprimerRolonyCountComparison.JPG]] ====Conclusions==== *The different RT primer sequences definitely have a consistent effect on number of primary rolonies that can be made **Most likely the 10nt primer sequence for 510nt and 1000nt matches some very high abundance transcripts (explore whether it matches ribosomal RNA here: [[Matt:LabNotes/2014-2-3]] *Since most primary rolonies aren't RAB7A no conclusions can be drawn whether distance from TSS affects CircLigase efficiency *Although there's only one set of RAB7A_1stRolonyFISH dye samples and the primary rolony counts are low, using targeted primers could still help increase yield of specific gene primary rolonies
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