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====Tagmentation==== # Add 2 ul HMW buffer to each PCR tube # Transfer 7ul of each sample to tubes #*PosCtrl: 1.06ng/ul #*NegCtrl: 0.884ng/ul #*Experimental: 0.818ng/ul #*NexteraControl (gDNA): 0.803ng/ul # Add 1 ul transposase (1:50 dilution) to each sample. Incubated 5.5 minutes at 55 degC # Dilute Qiagen protease 1:100 in H2O. Add 1 ul to each sample and incubate 10 minutes at 50 degC, followed by 20 minutes at 70 degC # Make the master mix for Exo- treatment: 1 ul Exo- and 0.4 ul dNTP per reaction # Add 1.4 ul Exo- mix to each sample and incubate 15 minutes at 37 degC, followed by 20 minutes at 75 degC
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