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===QIAEX-II Purification=== [http://www.qiagen.com/us/resources/download.aspx?id=13d33145-9f64-426a-a43b-394211d8cf2b&lang=en protocol] *Divide into 8 5mL tubes of 1200ul each *Add 3600ul (3X volume) of Buffer QX1 *Vortex the QIAEXII for 30sec *Add 30ul QIAEXII (30ul for every 10ug) *Incubate at 50C while mixing for 10min **Color should be yellow *Centrifuge sample for 30sec *Remove supernatant with a pipette *Wash the pellet with 500ul Buffer QX1 *Resuspend the pellet by vortexing *Transfer to 1.5ml tubes *Centrifuge sample for 30sec and remove supernatant again with pipette *Wash the pellet twice with 500ul Buffer PE *Air-dry the pellet for 30min until the pellet turns white *To elute, resuspend with 20ul H2O and vortexing *Incubate at RT for 5min *Centrifuge for 30sec and pipette out supernatant with sample *Repeat 20ul H2O resuspension and incubation to improve yield 10-15% *Total volume: 300ul *Nanodrop quantification: 155.7 ng/ul -> 46.7 ug
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