Editing
Matt:LabNotes/2016-9-9
(section)
Jump to navigation
Jump to search
Warning:
You are not logged in. Your IP address will be publicly visible if you make any edits. If you
log in
or
create an account
, your edits will be attributed to your username, along with other benefits.
Anti-spam check. Do
not
fill this in!
==Experimental Outline== #V8 Padlock Probe capture to RNA #*Ran out of V6 probes used last time #*V8 is a subset of V6 probes (targets constitutive exons instead of contigs of exons) #Quantify captured products by qPCR #*Also adds Illumina sequencing adapters ===Sample Groups=== *Use Universal Human Reference RNA (UHRR 740000-41) *3 RNA template concentrations for each (30ng, 150ng and 820ng) **For DNA template positive control only do 1 sample using 300ng gDNA 12878 #NTC - 8.19ng V8 - SplintR #NTC - 40.9ng V8 - SplintR #NTC - 221.1ng V8 - SplintR #30ng RNA - 8.19ng V8 - SplintR #150ng RNA - 40.9ng V8 - SplintR #820ng RNA - 221.1ng V8 - SplintR #PosCtrl: 300ng DNA - 16.38ng V8 - Ampligase #NegCtrl: 150ng RNA - 40.9ng V8 - Ampligase *Summary: **3 NTC samples with varying amount of padlock probes that matches experimental sample **3 experimental samples of 30ng, 150ng, and 820ng UHRR **1 PosCtrl that uses Ampligase for V8 to capture 300ng gDNA (876:1 probe:target ratio) **1 NegCtrl that uses Ampligase for V8 to capture 150ng UHRR
Summary:
Please note that all contributions to ZhangLabWiki may be edited, altered, or removed by other contributors. If you do not want your writing to be edited mercilessly, then do not submit it here.
You are also promising us that you wrote this yourself, or copied it from a public domain or similar free resource (see
ZhangLabWiki:Copyrights
for details).
Do not submit copyrighted work without permission!
Cancel
Editing help
(opens in new window)
Navigation menu
Personal tools
Not logged in
Talk
Contributions
Create account
Log in
Namespaces
Page
Discussion
English
Views
Read
Edit
View history
More
Search
Navigation
Main Page
Current events
Recent changes
Random page
Investigators
Matt Cai
Song Chen
Eric Chu
Dinh Diep
Elizabeth Duong
Shicheng Guo
Alan Fung
Daniel Jacobsen
Blue Lake
Huy Lam
Alice Li
Andrew Richards
Brandon Sos
Chris Wei
Yan Wu
Kun Zhang
Tools
What links here
Related changes
Special pages
Page information