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== Qubit ssDNA assay == * Follow protocol provided with the kit * Took 1ul of bis-cvt DNA into 9ul of H2O, then added the diluted DNA to 190ul ssDNA Qubit reagent (dilution factor = 200) {| {{table}} border = 1 | align="center" style="background:#f0f0f0;"|'''Sample ID''' | align="center" style="background:#f0f0f0;"|'''Well ID''' | align="center" style="background:#f0f0f0;"|'''Concentration in the Qubit (ng/mL)''' | align="center" style="background:#f0f0f0;"|'''Volume (ul)''' | align="center" style="background:#f0f0f0;"|'''Final conc. (ng/ul)''' | align="center" style="background:#f0f0f0;"|'''Yields (ng)''' |- | 1. H9||A1||76.40||17.00||15.28||252.12 |- | **2. H9+Activin 100ng/mL 5days||B1||62.70||17.00||12.54||206.91 |- | 3. H9+BMP4 50ng/mL 5days||C1||110.00||17.00||22.00||363.00 |- | 4. HUViPS4F1||D1||117.00||17.00||23.40||386.10 |- | 5. HUViPS4F1+Activin 100ng/mL 5days||E1||76.90||17.00||15.38||253.77 |- | 6. HUViPS4F1+BMP4 50ng/mL 5days||F1||114.00||17.00||22.80||376.20 |- | 7. HUViPS4F3||G1||118.00||17.00||23.60||389.40 |- | 8. HUViPS4F3+Activin 100ng/mL 5days||H1||79.30||17.00||15.86||261.69 |- | 9. HUViPS4F3+BMP4 50ng/mL 5days||A3||98.90||17.00||19.78||326.37 |- | GM11995 (wellID G7)||B3||205.00||17.00||41.00||676.50 |- | GM11993 (wellID F2)||C3||80.80||17.00||16.16||266.64 |- | **GM10861 (wellID A9)||D3||51.80||17.00||10.36||170.94 |- | GM11992 (wellID C2 )||E3||112.00||17.00||22.40||369.60 |- | GM11994 (wellID G9 )||F3||138.00||17.00||27.60||455.40 |- | GM10860 (wellID F10)||G3||116.00||17.00||23.20||382.80 |- | GM12813 (wellID E8)||H3||176.00||17.00||35.20||580.80 |- | GM12802 (wellID E7)||A5||133.00||17.00||26.60||438.90 |- | GM12815 (wellID G12)||B5||140.00||17.00||28.00||462.00 |- | GM12812 (wellID F11)||C5||112.00||17.00||22.40||369.60 |- | GM12814 (wellID A7)||D5||97.10||17.00||19.42||320.43 |- | GM12801 (wellID D9)||E5||122.00||17.00||24.40||402.60 |- | NA12156 (wellID B3)-1||F5||72.20||17.00||14.44||238.26 |- | NA12156 (wellID B3)-2||G5||68.40||17.00||13.68||225.72 |- | NA12156 (wellID B3)-3||H5||76.30||17.00||15.26||251.79 |- | NA12156 (wellID B3)-4||A7||85.30||17.00||17.06||281.49 |- | NA12156 (wellID B3)-5||B7||81.00||17.00||16.20||267.30 |- | NA12156 (wellID B3)-6||C7||85.20||17.00||17.04||281.16 |- | NA12156 (wellID B3)-7||D7||65.70||17.00||13.14||216.81 |- | NA12156 (wellID B3)-8||E7||80.10||17.00||16.02||264.33 |- | NA12156 (wellID B3)-9||F7||69.30||17.00||13.86||228.69 |- | NA12156 (wellID B3)-10||G7||68.90||17.00||13.78||227.37 |- | **2HFF P6||H7||44.00||17.00||8.80||145.20 |- | **218 HFF P7||A9||41.20||17.00||8.24||135.96 |- | KEBR028A (B4-plate2)-1,2||B9||38.50||17.00||7.70||127.05 |- | ****KEBR028A (tube)-1,2||C9||Out Of Range||17.00||#VALUE!||#VALUE! |- | KEBR040 (D4-plate1)||D9||157.00||17.00||31.40||518.10 |- | TZHZ018 (F1-plate1)||E9||137.00||17.00||27.40||452.10 |- | TZHZ031A (tube)-1,2||F9||274.00||17.00||54.80||904.20 |- | TZHZ041A (tube)-1,2||G9||215.00||17.00||43.00||709.50 |- | TZHZ075 (H01-plate2)-1,2||H9||164.00||17.00||32.80||541.20 |- | **PGP1iPS||A11||30.00||17.00||6.00||99.00 |- | PGP1iPS-IMPRINT||B11||42.90||17.00||8.58||141.57 |- | **Hues1||C11||35.80||17.00||7.16||118.14 |- | **Hues3||D11||34.60||17.00||6.92||114.18 |- | **Hues8||E11||43.40||17.00||8.68||143.22 |} * Continued on 2011_09_18: [[http://genome-tech.ucsd.edu/LabNotes/index.php/Noi/NOTES/2011-9-18]]
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