Editing
Noi/NOTES/2012-5-17
(section)
Jump to navigation
Jump to search
Warning:
You are not logged in. Your IP address will be publicly visible if you make any edits. If you
log in
or
create an account
, your edits will be attributed to your username, along with other benefits.
Anti-spam check. Do
not
fill this in!
= May 15, 2012, Cell pellet samples from Sergio to Dinh = {| {{table}} border = 1 | align="center" style="background:#f0f0f0;"|'''No.''' | align="center" style="background:#f0f0f0;"|'''Sample ID''' | align="center" style="background:#f0f0f0;"|'''Lab ID''' |- | ||'''hESC lines:'''|| |- | 1||H1 p48||Ma15-2 |- | 2||H9 p42 ||Ma15-3 |- | 3||HUES3 p33||Ma15-4 |- | 4||HUES6 p26||Ma15-5 |- | 5||HUES8 p35||Ma15-6 |- | 6||HUES9 p36||Ma15-7 |- | ||'''iPSC lines:'''|| |- | 7||ASThiPS4F3 p24 (derived from Astrocytes)||Ma15-8 |- | 8||ASThiPS4F4 p16 (derived from Astrocytes)||Ma15-9 |- | 9||FiPS4F2 p32 (derived from IMR90 fibroblasts)||Ma15-10 |- | 10||FiPS4F5 p29 (derived from IMR90 fibroblasts)||Ma15-11 |- | 11||KiPS4FA p54 (derived from keratinocytes)||Ma15-12 |- | 12||Huv-iPS4F6 p23 (derived from HUVECs)||Ma15-13 |- | ||'''Somatic lines:'''|| |- | 13||IMR90 p6||Ma15-14 |- | 14||Keratinocytes p3||Ma15-15 |- | ||'''Differentiation experiments:'''|| |- | 15||H1 p47 Control||Ma15-16 |- | 16||H1 p47 BMP-4||Ma15-17 |- | 17||ASThiPS4F5 p12 Control||Ma15-18 |- | 18||ASThiPS4F5 p12 BMP-4||Ma15-19 |- | 19||Huv-iPS4F6 p23 Control||Ma15-20 |- | 20||Huv-iPS4F6 p23 BMP-4||Ma15-21 |- | 21||Huv-iPS4F6 p23 ACTIVIN||Ma15-1 |- | ||Three samples below will not be captured with this batch|| |- | ||HUES2 p38|| |- | ||ASThiPS4F1 p8 (derived from Astrocytes) |- | ||ASThiPS4F2 p8 (derived from Astrocytes) |}<br> * '''gDNA extraction and elute with 250 buffer AE,(except sample Ap15-16 500ul)'''<br> {| {{table}} border = 1 | align="center" style="background:#f0f0f0;"|'''No.''' | align="center" style="background:#f0f0f0;"|'''Sample ID''' | align="center" style="background:#f0f0f0;"|'''Lab ID''' | align="center" style="background:#f0f0f0;"|'''Conc. (ng/ul)''' | align="center" style="background:#f0f0f0;"|'''Yields (ug) in 250ul''' |- | 1||HUES2 p41||Ap15-1||110.0||27.5 |- | 2||ASThiPS4F1 p12||Ap15-2||139.1||34.8 |- | 3||ASThiPS4F2 p12||Ap15-3||145.1||36.3 |- | 4||HUES6 p39 Control TeSR||Ap15-4||295.5||73.9 |- | 5||HUES6 p39 ACTIVIN||Ap15-5||128.1||32.0 |- | 6||HUES6 p39 BMP-4||Ap15-6||153.9||38.5 |- | 7||HUES9 p39 Control TeSR||Ap15-7||183.5||45.9 |- | 8||HUES9 p39 ACTIVIN||Ap15-8||149.3||37.3 |- | 9||HUES9 p39 BMP-4||Ap15-9||111.2||27.8 |- | 10||HUES3 p36 Control TeSR||Ap15-10||153.5||38.4 |- | 11||HUES3 p36 ACTIVIN||Ap15-11||87.5||21.9 |- | 12||HUES3 p36 BMP-4||Ap15-12||109.7||27.4 |- | 13||H1 p54 Control TeSR||Ap15-13||191.3||47.8 |- | 14||H1 p54 ACTIVIN||Ap15-14||108.7||27.2 |- | 15||ASThiPS4F5 p16 Control TeSR||Ap15-15||134.2||33.6 |- | *16||ASThiPS4F5 p16 ACTIVIN ||Ap15-16||50.2||25.1 |- | 17||ASThiPS4F4 p36 Control TeSR||Ap15-17||183.0||45.8 |- | 18||ASThiPS4F4 p36 ACTIVIN||Ap15-18||82.6||20.7 |- | 19||ASThiPS4F4 p36 BMP-4||Ap15-19||263.4||65.9 |}<br> * '''gDNA extraction and elute with 200 buffer AE,(except sample Ma15-14 150ul)'''<br> * Note: This batch I eluted with smaller volume (200ul except Ma1-14 which had very little of the cell pellet) of AE buffer because I was afraid that the concentration of some samples will be lower than 50ng/ul {| {{table}} border = 1 | align="center" style="background:#f0f0f0;"|'''No.''' | align="center" style="background:#f0f0f0;"|'''Sample ID''' | align="center" style="background:#f0f0f0;"|'''Lab ID''' | align="center" style="background:#f0f0f0;"|'''Conc. (ng/ul)''' | align="center" style="background:#f0f0f0;"|'''Yields (ug) in 200ul''' |- | 1||H1 p48||Ma15-2||215.2||43.0 |- | 2||H9 p47 ||Ma15-3||252.0||50.4 |- | 3||HUES3 p33||Ma15-4||226.1||45.2 |- | 4||HUES6 p26||Ma15-5||238.7||47.7 |- | 5||HUES8 p35||Ma15-6||83.4||16.7 |- | 6||HUES9 p36||Ma15-7||236.8||47.4 |- | 7||ASThiPS4F3 p24 (derived from Astrocytes)||Ma15-8||175.9||35.2 |- | 8||ASThiPS4F4 p16 (derived from Astrocytes)||Ma15-9||164.3||32.9 |- | 9||FiPS4F2 p32 (derived from IMR90 fibroblasts)||Ma15-10||205.9||41.2 |- | 10||FiPS4F5 p29 (derived from IMR90 fibroblasts)||Ma15-11||97.6||19.5 |- | 11||KiPS4FA p54 (derived from keratinocytes)||Ma15-12||129.5||25.9 |- | 12||Huv-iPS4F6 p23 (derived from HUVECs)||Ma15-13||263.1||52.6 |- | *13||IMR90 p6 (eluted with 150ul AE buffer)||Ma15-14||76.1||11.4 |- | 14||Keratinocytes p3||Ma15-15||138.7||27.7 |- | 15||H1 p47 Control||Ma15-16||216.4||43.3 |- | 16||H1 p47 BMP-4||Ma15-17||137.6||27.5 |- | 17||ASThiPS4F5 p12 Control||Ma15-18||224.6||44.9 |- | 18||ASThiPS4F5 p12 BMP-4||Ma15-19||111.7||22.3 |- | 19||Huv-iPS4F6 p23 Control||Ma15-20||289.9||58.0 |- | 20||Huv-iPS4F6 p23 BMP-4||Ma15-21||172.9||34.6 |- | 21||Huv-iPS4F6 p23 ACTIVIN||Ma15-1||86.7||17.3 |} * Note: All samples above have 260/280 between 1.98-2.07 * Continued on: http://genome-tech.ucsd.edu/LabNotes/index.php/Noi/NOTES/2012-5-20
Summary:
Please note that all contributions to ZhangLabWiki may be edited, altered, or removed by other contributors. If you do not want your writing to be edited mercilessly, then do not submit it here.
You are also promising us that you wrote this yourself, or copied it from a public domain or similar free resource (see
ZhangLabWiki:Copyrights
for details).
Do not submit copyrighted work without permission!
Cancel
Editing help
(opens in new window)
Navigation menu
Personal tools
Not logged in
Talk
Contributions
Create account
Log in
Namespaces
Page
Discussion
English
Views
Read
Edit
View history
More
Search
Navigation
Main Page
Current events
Recent changes
Random page
Investigators
Matt Cai
Song Chen
Eric Chu
Dinh Diep
Elizabeth Duong
Shicheng Guo
Alan Fung
Daniel Jacobsen
Blue Lake
Huy Lam
Alice Li
Andrew Richards
Brandon Sos
Chris Wei
Yan Wu
Kun Zhang
Tools
What links here
Related changes
Special pages
Page information