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== epMotion set up == * CSV input files (CSV formatted is recommended to be imported to the protocol, epMotion5075) # [[Media:AC-777_plate1_A1-H12.csv| AC-777_plate1_A1-H12.csv]] # [[Media:AC-777_plate2_I1-P12.csv| AC-777_plate2_I1-P12.csv]] # [[Media:AC-777_plate3_A13-H24.csv| AC-777_plate3_A13-H24.csv]] # [[Media:AC-777_plate4_I13-P24.csv| AC-777_plate4_I13-P24.csv]] * Note: # I prefer to split CSV file into 4 files to prevent mis-transferring of sample to the wrong plates. # I set up a larger sample volume (15ul) than the actual volume to make sure all liquid is transferred to the new plate The tool numbers in the CSV file must match the dispensing tools as follows: 1 - TS_50 2 - TS_300 * Note about DNA transfer from 384-well plate to 96-well plate and from 96well plate to Covaris shearing plate (will be filled later) * 2013/01/14 '''Set up for Covaris shearing 400bp (total volume 50ul in H2O)''' Target BP 400 Tube Micro Duty Factor 10% Peak Incident Power (w) 140 Cycle per Burst 200 Time (secs) 55 Volume 50ul Temperature (c) 7 Intensifier Yes '''Set up for Covaris shearing 2000bp from Chris (PacBio recommendation)''' '''Note:''' This will be adjusted later based on the recommendation of the machine. Need to use "'Mini-tube Clear''' Target Shear Size: 2.0kb Tube: Mini-tube Clear Shearing Volume: 200.0ul (used total volume 50ul) Temp of Chiller: 4C Temp of Bath: 6-8C Duty Cycle: 20 Intensity: 0.1 --> convert to Peak Incident Power (w): 3.5 Cycles per Burst: 1000 Time: 15cycles x 60sec -> this mean it takes 15min to shear 1 sample Amount of DNA in Tube: 2ug-20ug * Transferred sample to striptube and handed to Chris * Continued on: http://genome-tech.ucsd.edu/LabNotes/index.php/Noi/NOTES/2013-1-15
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