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===Expansion PCR=== ====Quick run with 1ul for each primer set==== {| {{table}} border=1 | align="center" style="background:#f0f0f0;"|'''Tube ID''' | align="center" style="background:#f0f0f0;"|'''Sample''' | align="center" style="background:#f0f0f0;"|'''[nM]''' | align="center" style="background:#f0f0f0;"|'''[ul]''' | align="center" style="background:#f0f0f0;"|'''AmpP''' | align="center" style="background:#f0f0f0;"|'''Ct up''' | align="center" style="background:#f0f0f0;"|'''Ct stop''' | align="center" style="background:#f0f0f0;"|'''final Flu.''' |- | s1||T21.v4 ePCR||361||0.5||v4 **||1||6||0.32 |- | s2||oligo mix||~ 169||1||v4 **||13||20||~0.2 |- | s3||T21.v6 ePCR||181||1||v6||1||7||0.28 |- | s4||oligo mix||~105||1||v6||15||20||~0.13 |- | s5||selector ePCR||150||1||eMIP||no||20||no |- | s6||oligo mix||~226||1||eMIP||no||20||no |} [[File:ePCR_QPCR.jpg|400px]][[File:ePCR_PAGE.jpg|400px]] ====Recheck with eMIP primer sets==== * Positive control used is the pPCR product (65nM is confirmed by Nanodrop again) of selector set last time * Probe/primer were also confirmed with PAGE together with PCR product * The problem may be due to eMIP primer set -> re-order [[File:quickRun_eMIP_QPCR.jpg|400px]][[File:quickRun_eMIP_PAGE.jpg|400px]] * Fortunately, I found another eMIP pair from my box, so I gave this set a try with PC used above. * It works as expected, so I toss the bad primers originally in Noi's box. [[File:ePCR_eMIP_QPCR.jpg|400px]][[File:ePCR_v4.jpg|400px]][[File:quickRun_PAGE.jpg|400px]][[File:ePCR_eMIP.jpg|400px]]
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