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===Results=== *Each samples (tube1 to 8) have 4 aliquots for PCR, 40ul/aliquotes *4ul PCR products from one aliquot per sample was checked by 6% TBE-PAGE *30min running time '''Sample arrangement''' Template P1 primer SSIII Exo buffer ExoI Final Tube1 water 0.125ul 0.33ul 1* in 1ul 0.5U 0.16* in 6ul Tube2 water 0.125ul 0.33ul 1* in 1ul 2U 0.16* in 6ul Tube3 water 0.125ul 0.33ul 3* in 1ul 4U 0.5* in 6ul Tube4 water 0.125ul 0.33ul 6* in 1ul 8U 1* in 6ul Tube5 0.1pg 6-EB cDNA 1* in 1ul 0.5U 0.16* in 6ul Tube6 0.1pg 6-EB cDNA 1* in 1ul 2U 0.16* in 6ul Tube7 0.1pg 6-EB cDNA 3* in 1ul 4U 0.5* in 6ul Tube8 0.1pg 6-EB cDNA 6* in 1ul 8U 1* in 6ul [[File:Exo I.jpg]]
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