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==Procedures (Adapted from Jeff's note)== ===Slide washing (Tween 20/PBS washing) === #Add 20 uL of Tween 20 to 180 uL of H20 (10% dilution). #Add 19.2 mL of PBS to the solution for a final dilution of .1% Tween 20. #Shake until a soapy consistency is observed #Gently spray 5 ml of the solution over the microarrays #Wipe carefully with a kimwipe and allow to dry ===Gasket Application=== #Remove the protective covering from one side of the gasket and place in the desired position on the microarray #Firmly press down until the squares are sealed #Remove the protective covering from the other side and press down, ensuring a tight seal === Bead Loading === #Dilute the beads so that the concentration is 1mg bead/200 uL washing buffer (6-7 x 10^7 beads in solution) #Do another 1:200 dilution (1500 beads) and a 1:100 dilution (3000 beads) #Place the microarray over a magnetic bar #Add 15 uL beads from each dilution to 2 squares each #Dry for 10 min (or until the water has fully evaporated)
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