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==Procedures== ===QUBIT quantification=== [[File:sam111710-qubit-new.jpg|900px]] *IC = internal control of E.coli gDNA *4 pairs of samples(marked in red) were prepared for shearing: **WGA102710-(2-1)- 3hr and 10hr **WGA102710-(2-4)- 3hr and 10hr **WGA110310-(2)- 4hr and 10hr **WGA110310-(4)- 4hr and 10hr ===Sample preparation=== *Label the Covaris microtubes. Open the caps and leave tubes on 96-well rack. *UV-treat the tubes and pipettes for 15min. **Thaw the tRNA carrier (stored in -80C). Use the "2"-tRNA(96ng/uL) Sample no. Sample name Conc.(ng/uL) Sample(uL) tRNA("2") H2O Total DNA(ng) --------------------------------------------------------------------------------------------------- HC01 WGA102710-(2-1)- 3hr 0.88 50.00 3.5 0.00 43.95ng HC02 WGA102710-(2-1)-10hr 729.07 0.68 - 49.00 500.00ng HC03 WGA102710-(2-4)- 3hr 0.98 50.00 3.5 0.00 48.95ng HC04 WGA102710-(2-4)-10hr 693.02 0.72 - 49.00 500.00ng HC05 WGA110310- (2) - 4hr 2.16 50.00 - 0.00 108.14ng HC06 WGA110310- (2) -10hr 661.63 0.75 - 49.00 500.00ng HC07 WGA110310- (4) - 4hr 3.09 50.00 - 0.00 154.65ng HC08 WGA110310- (4) -10hr 974.42 0.52 - 49.00 500.00ng *I didn't put any tRNA carrier for 4hr-amplified amplicons. *'''50uL''' sample suspension were carefully transferred into microtubes. *Samples tubes were tapped on the rack and packed in a paper box with three disposable cooler. *The package was shipped to the Sara through FedEx 11-17-'10 by Alice.
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