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Tina:Cardiomyocyte differentiation in H9 ESCs (CDH9)
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==Experiment #3 (CDH9_E1_T3)== *Study Purpose: Increase cell number to see if thing goes better. *Date: 5/1/2013 ~ 5/28/2013 *Cells: H9 ESC line, parental cell (without brainbow construct in it). Cells seeded on 5/1. ('''1:6''' dilution from feeder). *Protocol used: Christian Metallo Lab protocol. *Two induction medium used: Gln+ induction medium,DMK induction medium *Cell density used: 100% confluency. '''6-well plate'''<br> Well #1: 100% confluency, DMK induction medium, repeat 1 Well #2: 100% confluency, Gln+ induction medium, repeat 1 Well #3: 100% confluency, Gln+ induction medium, repeat 2 Well #4: 100% confluency, DMK induction medium, repeat 2 *Induction: 5/6 (Day 0). Cells reached 100% confluency. Start experiment. Add medium first and then add CHIR99021 and DMK directly into each well. *5/7 (Day 1). Change medium (pre-mix DMK). Still, observed lots of cell round-up. But much lesser than before. I can still see many cells attached to the dish this time. *5/8 ~ 5/28. Change medium accordingly. Take pictures. *Cells differentiate extremely well in 6-well plate. *Pictures: *Movies on 5/27/2013 (Day 21): *Flow analysis:
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