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==Conclusion== *Due to bad experimental design with no positive and negative control, I obtained none useful information through this experiment. *No +ve control, *No -ve control *It could be the adapter annealing problem (solved by +ve control + qpcr after ligation) *It could be the self annealing of adapter by the adapter *The primer used for amplification of methylation adapter might not be the right one.(update primer need to be used is the PCR primer PR 1.0 and PCR primer PE2.0 which is the one I used PCR_F and PCR_R)
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