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==MDA Analysis== [[File:ZhangLab_2 2011-09-08 11hr 32min.jpg|thumb|500px]] *Denature all samples at 95C for 30sec and quickly transfer to cooler rack {| {{table}} | align="center" style="background:#f0f0f0;"|'''Content''' | align="center" style="background:#f0f0f0;"|'''Volume per Reaction''' | align="center" style="background:#f0f0f0;"|'''MM''' |- | 10X Repli Phi Buffer||2||8.8 |- | 1mM N9 Primer||1||4.4 |- | 25mM dNTP||0.8||3.52 |- | Repliphi phi 29 (100U/ul)||1||4.4 |- | Klenow Exo Minus (10U/ul)||0.5||2.2 |- | 50X SYBG||0.1||0.44 |- | H2O||9.6||42.24 |- | Modified DNA Sample||5||Do not add to MM |- | Total||20||66 |} *Add 15ul of MDA mastermix to each sample *30C 120 min, 65C 10 min, 15C hold
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