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Andy Chen:Micromanipulation
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===Tips=== *Tips around 20-50 μm in diameter are ideal. *When filling a dry microwell with medium, there seems to be a threshold to getting the medium out of the tip. Thus positive pressure needs to be applied carefully to prevent flooding and possible contamination. Pressure should be increased slowly. If there appears to be a bulge of medium on the tip, try to move the tip around on the bottom of the well to get some liquid into the well, making it easier to add medium. *I found it easier to move to the general location of the well using the [0] setting, then changing it to [1] for finer movements. [0] seems to be affected by acceleration, so if you aren't careful, it is easy to over move it and smash the tip into the well.
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