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====Plate prep==== * Each plate has 16 arrays, each of which is independent (separated by rubber gasket). Each array has ~150 wells. * Wells are 400 um ID, 100 um in height. * Do not transport the plate uncovered outside the hood. # Plate must be O2 plasma treated in Nano3 facility at Calit2 immediately prior to BSA coat. Treatment lasts less than 1 hour. # Wipe down hood, pipettes, and tip boxes with EtOH, then DNA away, then UV. # UV treat the plate after plasma treatment. # Add 10 ul 1% BSA to each array. Be careful to coat entire array. Vacuum 30 seconds in vacuum centrifuge, then UV treat and let sit 1 hour at RT. # Wash 3x with 10 ul PBS. Leave the last wash on. # Vacuum 30 seconds in vacuum centrifuge. # Remove last wash. Vacuum again 30 seconds. # UV treat 10 min.
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