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== C1 Loading == :*Medium Chip was primed :*Nuclei and cells were mixed in a 1:1 ratio (7.5ul huNu/7.5ul mESC/10ul C1 cell suspension RGT) for the following total number loaded: :**~2000 huNu :**~1000 mESCs :*Note: these numbers were chosen based on the fact that the previous C1 loading of ~750 huNu only resulted in 33 capture sites and that the mESC expected size was ~9um which would require 2000-3000 for efficient loading. Therefore we used a little over twice the number of nuclei and "spiked-in" mESCs at a sub-optimal loading quantity that would be expected to achieve a low number of capture sites.
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