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===Results=== *total RNA. {| {{table}} | align="center" style="background:#f0f0f0;"|'''10/7/2015''' | align="center" style="background:#f0f0f0;"|'''intensity''' | align="center" style="background:#f0f0f0;"|'''ng/ul''' | align="center" style="background:#f0f0f0;"|'''''' | align="center" style="background:#f0f0f0;"|'''''' | align="center" style="background:#f0f0f0;"|'''''' | align="center" style="background:#f0f0f0;"|'''''' |- | ||1763.757444||5|||||||| |- | ||895.2045194||2.5||in 10 uL||||for 50 ng||H2O |- | 1. T7-nXTv2_i5-1, 5X tn5059, 539 cells, buffer D6 Rep1||33570.21118||96.55013139||965.5013139||||0.517865686||8.482134314 |- | 2. T7-nXTv2_i5-1, 5X tn5059, 539 cells, buffer D6 Rep2||15673.5593||45.03728923||450.3728923||||1.110191152||7.889808848 |- | 3. T7-nXTv2_i5-1, 5X tn5059, 539 cells, buffer D6 Rep3||25727.79598||73.97690936||739.7690936||||0.675886576||8.324113424 |- | 4. T7-nXTv2_i5-1, 5X tn5059, 539 cells, buffer TA Rep1||28224.6736||81.16379904||811.6379904||||0.616038192||8.383961808 |- | 5. T7-nXTv2_i5-1, 5X tn5059, 539 cells, buffer TA Rep2||36786.24018||105.806991||1058.06991||||0.472558567||8.527441433 |- | 6. T7-nXTv2_i5-1, 5X tn5059, 539 cells, buffer TA Rep3||35388.89339||101.7849367||1017.849367||||0.491231823||8.508768177 |- | 7. T7-nXTv2_i5-1, 5X tn5059, 6ng DNA, buffer D6||108457.5135||312.1020574||3121.020574||/4||0.640816026||8.359183974 |- | 8. T7-nXTv2_i5-1, 5X tn5059, NTC, buffer D6||189.5401283||0.468850339||4.688503394||1||||9 |- | |} *TBU gel after IVT, Guanidine HCl to remove proteins [[File:ZhangLab 2 2015-10-05 15hr 05min-labeled.jpg|600px]] *qPCR curves [[File:2015-10-07 hiscribe TA v D6 comparison.bmp|600px]] *TBE after PCR amplification [[File:ZhangLab 2 2015-10-07 16hr 32min-labeled.jpg|600px]] *gel size selection amounts {| {{table}} | align="center" style="background:#f0f0f0;"|'''10/7/2015 gel size selection''' | align="center" style="background:#f0f0f0;"|'''''' | align="center" style="background:#f0f0f0;"|'''''' | align="center" style="background:#f0f0f0;"|'''''' | align="center" style="background:#f0f0f0;"|'''''' |- | ||5344.98624||5|||| |- | ||2976.702589||2.5||for 50 ng||X2 |- | 1. T7-nXTv2_i5-1, 5X tn5059, 539 cells, buffer D6 Rep1||21574.08855||22.13171298||2.259201538||4.518403075 |- | 2. T7-nXTv2_i5-1, 5X tn5059, 539 cells, buffer D6 Rep2||25469.0217||26.24326983||1.905250387||3.810500774 |- | 3. T7-nXTv2_i5-1, 5X tn5059, 539 cells, buffer D6 Rep3||14060.63573||14.20038598||3.521031052||7.042062104 |- | 4. T7-nXTv2_i5-1, 5X tn5059, 539 cells, buffer TA Rep1||12155.62807||12.1894279||4.101915233||8.203830466 |- | 5. T7-nXTv2_i5-1, 5X tn5059, 539 cells, buffer TA Rep2||10532.47809||10.47600352||4.772812446||9.545624893 |- | 6. T7-nXTv2_i5-1, 5X tn5059, 539 cells, buffer TA Rep3||9135.697926||9.001538924||5.554605765||11.10921153 |- | 7. T7-nXTv2_i5-1, 5X tn5059, 6ng DNA, buffer D6||24780.47545||25.51642884||0||0 |- | 8. T7-nXTv2_i5-1, 5X tn5059, NTC, buffer D6||-143.1827782||-0.793403394||1||2 |- | |||||||| |- | |} *gel size selection [[File:ZhangLab 2 2015-10-07 18hr 09min-labeled.jpg|600px]] *gel size selection validation *ran on TBU cuz no TBEs were available. looks fine. [[File:ZhangLab 2 2015-10-08 11hr 17min-labeled.jpg|600px]]
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