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===Results=== *total RNA. Used 1200 intensity for 2.5 ng ladder band since its intensity was a littler higher then the 5 ng band. should be +/- 10 ng then. {| {{table}} | align="center" style="background:#f0f0f0;"|'''''' | align="center" style="background:#f0f0f0;"|'''intensity''' | align="center" style="background:#f0f0f0;"|'''ng/ul''' |- | ||2244.490067||5 |- | ||1200||2.5 |- | 1. t7-top2, 5X tn5-059, 100 cells rep1||10083.3803||23.76248153 |- | 2. t7-top2, 5X tn5-059, 100 cells rep2||9901.370295||23.32683831 |- | 3. t7-top2, 5X tn5-059, 1000 cells||47608.67142||113.5797338 |- | 4. t7-top2, 5X tn5-059, NTC||2298.250068||5.128675234 |- | 5. t7-top2, 5X tn5-059, 6 ng pure DNA||165223.0349||395.0911795 |- | |} *TBU gel after IVT, Guanidine HCl to remove proteins [[File:ZhangLab 2 2015-03-24 14hr 42min-labeled.jpg|600px]] [[File:ZhangLab 2 2015-03-24 14hr 44min-invert-labeled.jpg|600px]] '''3/25/2015 UPDATE AFTER PCR ADDITION OF BARCODES''' *In total ran for 12 cycles, curves came up later then usual maybe since miscalculated and added less RNA. *qPCR curves [[File:2015-03-25 100 cells test, barcode addition.bmp|600px]] *after adding barcodes, TBE gel [[File:ZhangLab 2 2015-03-25 18hr 58min-labeled.jpg|600px]] [[File:ZhangLab 2 2015-03-25 18hr 59min-labeled.jpg|600px]] *gel size selection, 220-800 ish bp [[File:ZhangLab 2 2015-03-26 13hr 13min-labeled.jpg|600px]] *gel size selection validation [[File:ZhangLab 2 2015-03-26 17hr 10min-labeled.jpg|600px]]
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