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===Results=== *total RNA. {| {{table}} | align="center" style="background:#f0f0f0;"|'''''' | align="center" style="background:#f0f0f0;"|'''changed to 4000 since to close''' | align="center" style="background:#f0f0f0;"|'''''' | align="center" style="background:#f0f0f0;"|'''''' | align="center" style="background:#f0f0f0;"|'''''' | align="center" style="background:#f0f0f0;"|'''''' |- | 4/1/2015 RNA calcs||4000||5|||||| |- | ||2180.400065||2.5||for 50 ng||||H20 for 10 uL |- | 1. idx49, t7-top2, 5X tn5-059, 500 cells rep1||35668.54106||48.51030748||1.030708783||||8.969291217 |- | 2. idx50, t7-top2, 5X tn5-059, 500 cells rep2||37453.28112||50.9624125||0.981115249||||9.018884751 |- | 3. idx51, t7-top2, 5X ez-tn5, 500 cells rep1||112276.7833||153.7645461||1.300689952||/4||8.699310048 |- | 4. idx52, t7-top2, 5X ez-tn5, 500 cells rep2||129529.0439||177.4679165||1.126964265||/4||8.873035735 |- | 5. idx53, t7-top2, 5X tn5-059, 6 ng pure DNA||216604.4465||297.1032838||1.34633315||/8||8.65366685 |- | 6. idx54, t7-top2, 5X ez-tn5, 6 ng pure DNA||213967.8664||293.4808115||1.362951117||/8||8.637048883 |- | 7. idx55, t7-top2, 5X tn5-059, NTC||64.53000192||-0.407053939||1||||9 |- | 8. idx56, t7-top2, 5X ez-tn5, NTC||0||-0.49571354||1||||9 |- | |} *TBU gel after IVT, Guanidine HCl to remove proteins [[File:ZhangLab 2 2015-04-01 15hr 26min-labeled.jpg|600px]] [[File:ZhangLab 2 2015-04-01 15hr 28min-invert-labeled.jpg|600px]] *qPCR curves [[File:2015-04-02 500 cells tn5-059 vs ez tn5.bmp|600px]] *after adding barcodes, TBE gel [[File:ZhangLab 2 2015-04-02 14hr 22min-labeled.jpg|600px]] *gel size selection amounts {| {{table}} | align="center" style="background:#f0f0f0;"|'''gel size selection amounts''' | align="center" style="background:#f0f0f0;"|'''''' | align="center" style="background:#f0f0f0;"|'''''' | align="center" style="background:#f0f0f0;"|'''''' | align="center" style="background:#f0f0f0;"|'''''' |- | 4/2/2015||intensity||ng/ul|||| |- | ||7213.490215||5|||| |- | ||5410.760161||2.5||for 80 ng|| |- | 1. idx49, t7-top2, 5X tn5-059, 500 cells rep1||19053.90057||21.42011006||3.734808075|| |- | 2. idx50, t7-top2, 5X tn5-059, 500 cells rep2||16376.87049||17.70764341||4.517823076|| |- | 3. idx51, t7-top2, 5X ez-tn5, 500 cells rep1||18371.57055||20.47386464||3.907420578|| |- | 4. idx52, t7-top2, 5X ez-tn5, 500 cells rep2||16685.3305||18.13541129||4.411259205|| |- | 5. idx53, t7-top2, 5X tn5-059, 6 ng pure DNA||38879.07116||48.91337028||1.635544628||0.8 uL |- | 6. idx54, t7-top2, 5X ez-tn5, 6 ng pure DNA||34297.93102||42.56031131||1.879685499||0.8 uL |- | 7. idx55, t7-top2, 5X tn5-059, NTC||-2643.330079||-8.669296012||-9.227969594||1 uL |- | 8. idx56, t7-top2, 5X ez-tn5, NTC||-1150.120034||-6.59853389||-12.1239053||1 uL |- | |} *gel size selection, 220-800 ish bp [[File:ZhangLab 2 2015-04-02 16hr 21min-labeled.jpg|600px]] *gel size selection validation [[File:ZhangLab 2 2015-04-02 19hr 42min-labeled.jpg|600px]]
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