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===Calculation of mass of probe required for each human gDNA input=== *Can find calculation on my Google Doc at <https://docs.google.com/spreadsheets/d/10hhMyThBT2nAvxIodQq_1q0RQopnfMlNzSEZEexi0nE/edit?usp=sharing> *For each of the tests, the following is the probe and target masses required: rxn Target (ng) Probe:Target Probe (ng) 1 50ng 1:200 1.65 2 50ng 1:300 2.48 3 50ng 1:400 3.30 4 100ng 1:200 3.30 5 100ng 1:300 4.95 6 100ng 1:400 6.60 7 200ng 1:200 6.60 8 200ng 1:300 9.90 9 200ng 1:400 13.20 *Dilute probe mix to 2ng probe/ul **Probe concentration is ~50ng/ul -> 1/25d -> 1ul probe + 24ul water *Dilute target human gDNA concentration to 20ng/ul **gDNA concentration is ~50ng/ul -> 1/2.5d -> 20ul gDNA + 30ul water) *Final concentration of probe and target to add to each rxn rxn Target (ul) Probe (ul) Water (ul) -> to get to 20ul total volume 1 2.5 0.825 16.675 2 2.5 1.24 16.26 3 2.5 1.65 15.85 4 5.0 1.65 13.35 5 5.0 2.48 12.525 6 5.0 3.30 11.7 7 10.0 3.30 6.7 8 10.0 4.95 5.05 9 10.0 6.60 3.4
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