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===C1 DNA-seq=== *For most of the experiment, we used the published C1 DNA-seq protocol: [[File:C1_DNA_Seq_protocol_100-7135.pdf]] *The C1 protocol suggests to wash cells 2 times with C1 DNA Seq Cell Wash Buffer. For this run, we only washed the cells once using the following procedure: 1) Add wash buffer (with 1% BSA added) to 100ul (95ul wash + 5ul sample) 2) Spin down (300g 5min) 3) Remove all but 5ul 4) Prepare cell mix by adding 3.5ul cells + 1.5ul suspension reagent *Once the C1 finished running, utilized the PicoGreen assay as suggested by Fluidigm to quantify all the samples at once. Below is the template and results for this assay **Template: [[File:C1Dna2014Jul23PicoGreenTemplate.xlsx]] **Results: [[File:2014_07_30_results-C1Dna2014Jul23PicoGreen.xlsx]] **As seen in the above results, the concentration seems a bit lower than expected (should expect ~8-15ng/ul = 100ng output based previous discussion with Fluidigm representative)
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