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===Prep KLN mix solution (6 strips, 8 rxn's each)=== *We are using HemloKlenTaq for the reaction (because AmpliTaq Stoffel was discontinued) *First, perform the following dilutions: **Dilute dNTP 1/10d (6ul dNTP + 54ul H2O) (10mM/nt -> 1mM/nt) *Mastermix recipe Component Stock conc Final conc 1x(ul) MM Vol (54x) (ul) Hemo KlenTaq 100% 20% 0.4 21.6 AmpLigase 5U/ul 0.5U/ul 0.2 10.8 dNTP 1mM 100uM 0.2 10.8 10x AmpLigase Buff 10x 1x 0.2 10.8 H2O 1.0 54 *Aliquot 13ul KLN mix to each well of a strip tube so can use multichannel when adding to the reactions *Add 2ul KLN mix to all strips (columsn 1->6) using multichannel
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