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===Discussion=== Well, this pretty much confirms it. AmpF is the problem and is producing off-target products. Note that the expected product is the 168 bp band, but a primer dimer caused by the off-target binding would produce a product of approximately 75bp. We see a clear band at 75 bp, meaning that the AmpF-primer2 binding is likely too strong. Hopefully with changing primer2 to primer24 (see [[Daniel:Notebook/ComboLock/2016-9-28|PCAmp4 production]]) for further details.
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